Fig. 6. Pra-B inhibits the TPA-induced IKKα/NF-κB signaling pathway in HeLa cells. The cells were pretreated with TPA (50 ng/ml) for 2 h and then incubated with Pra-B (10 and 20 μM) for 24 h. Nuclear and cytosolic proteins were resolved and analyze the levels of NF-κB-p65/-p50. Lamin B and α-tubulin were used as internal controls for nuclear and cytosolic fractions, respectively. (B) Cells were treated as mentioned above, and protein expression of p-IKKα and IKKα was measured through Western blotting. β-actin was used as an internal control. (C) HeLa cells were cotreated with TPA and Pra-B in the absence or presence of LY294002 for 24 h, and the nuclear expression of NF-κB-p65/-p50 was measured through Western blotting. These results are representative of three independent experiments. **P<0.01 compared with untreated cells. #P<0.01 compared with TPA alone.